The BioInformatics engine for Genetic Data
KBioBox is a technology company with expertsie in BioInformatics specialising in fast precision off target analysis and gene edit biodesign. Created in reponse to the growing need for real-time genetic sequence analysis, KBioBox's BioEngine is a powerful genomic search engine capable of searching entire genomes in minutes for even the most complex searches. BioEngine technology is build upon novel proprietary search algorythms that do not compromise speed for accuracy. Simple interactiveness and minimal computation requirements mean the technology can be used in the field or in the cloud.
Genetic Data, KBioBox's BioEngine
The bio-informatics engine providing speed without compromising accuracy, adaptable to any bio-informatics application.
KBioBox's BioEngine empowers research and provides improved quality for your application.
Technologies
Off Target Analysis
Throughly check your gene edit for potential off target interactions in under a minute. Know where to check to ensure a clean edit.
Gene Edit Design
Rapidly calculate the "top ten" targets for your gene edit via off target potential consideration. Each calculated target includes a full off target analysis.
Pathogen Identification/Analysis
Compare assembled or raw sequence data against millions of references in minutes, view alignments and homologies
De Novo Assembly
Our unique technology affords new approaches to this old problem
Microbiome Analysis
Separate, assemble, identify, and annotate.
Multi-Species Alignment
Discover new relations between species, identifying similar and conserved regions.
Custom applications
Connect your application to KBioBox's BioEngine to handle the searching, and you to handle the analysis
Design
Use BioEngine technology to design optimal guides for one target or across multiple targets and species. Targets and guides are determined based on minimal off target interaction.
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Predict
Already have a target and guide? Check across one or more species for potential off target sites. Each off target report includes annotation and primer information.
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Check
After a gene edit use KBioBox's extended GUIDE-Seq analysis, extracting cleavage site and frequency, correct/incorrect repairs, and translocation information from GUIDE-seq or similar pipelines.
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Application Areas
Bio-Technology
Gene Editing
Bio-Agriculture
Cell Line Engineering
KBioBox's BioDesign tool and Off Target Analysis tool mean better, more stable lines, and provide quality check points
Off Target Analysis
Identify potential off target locations quickly and accurately on any genome, optional primer design calculation.
Plant Engineering
With either novel gene editing or traditional cross pollination techniques, KBioBox technology provides the best path towards success.
Microbiome
Rapidly identify all species present in a sample, accurate to the subspecies level
Target Design
An inversion of KBioBox's excellent Off Target Analysis, let the Bio-Engine design your guides.
Soil Microbiome
Assemble and identify known species, flag and mark unknown species
Diagnostics
Quickly compare against millions of pathogen references, identifying with subspecies accuracy
"Extended" GUIDEseq
Extract additional information from the the GUIDEseq protocol, including cleavage sites/sizes and translocations.
Pathogen Tracking
Identify and track pathogens and unique subspecies over time
De Novo Assembly
KBioBox's patented technology provides a novel high performing approach to assembly.
CAR T-Cell engineering
Use KBioBox's powerful tools to design, check, and ensure T Cell engineering.
Oncology
Compare across references or search for markers, include variant analysis to determine potential sites
Annotations
Forward or reverse quickly find and label
Technical Applications
BioEngine Technology
KBioBox's technology is simple to interact with and can power any bioinformatics application, decreasing search time without sacrificing accuracy
Web API
Harness the power of the BioEngine in house to accelerate research or embedded on your website to better serve your customers
Empower existing informatics
Augment and enhance existing programs and protocols with the power of KBioBox's BioEngine
Cloud BioEngine
Deploy KBioBox's BioEngine into your cloud, or interact through KBioBox's cloud
A Performance Example
KBioBox's BioEngine applied to Gene Editing Off Target Analysis
KBioBox's Off Target Analysis application predicted 96.6% of off-targets found by GUIDE-seq, the MIT and E-CRISP tools found less then 50%.
On average KBioBox's Off Target Analysis ran in under two minutes, less than half the time for the MIT and E-CRISP tools.
Built upon KBioBox's BioEngine, the Off-Target Analysis tool predicted 96.6% of off-targets reported by the GUIDE-seq method in under two minutes. By comparison MIT's CRISPR design tool, and the E-CRISP tool both found less than 50% of total off-targets reported by GUIDE-seq.
Roles
Analysing NGS data, design a gene edit, or identifying unknown genetic material, KBioBox's BioEngine provides the computational power to expedite projects, tests, and research
Cell Line Scientist
KBioBox's BioEngines tedious attention to detail empowers scientists to develop better more stable cell lines the first time. The BioDesign tool narrows possible targets into the "top ten", while the Off Target Analysis tool highlights loci to check after a gene edit.
Genomicist
Identifying and tracking variants across multiple species is a daunting task. KBioBox's BioEngine accelerates and simplies this task, alleviating scientists for more important tasks.
Gene Editing Specialist
Easily customize KBioBox's BioEngine for any chemistry or combination of chemistries. Then let the BioEngine handle the tedious checking and accounting to ensure a successful gene edit.
Bioinformaticist
Enhance existing bioinformatics pipelines with KBioBox's BioEngine, greatly improving search speed and capabilities. Complete research and projects expedited, allowing scientists to spend more time on important tasks.
Plant Genome / Editing Specialist
Coax every last drop of information or build an exact genomic map of your plant, for plant design or traditional cross breeding efforts. KBioBox's tools preserve all polypoidal information.
Vaccine Scientist / Oncologist
Whether researching cancer, developing cancer vaccines, or pushing the boundaries of modern vaccination techniques, KBioBox's tools provide the most robust, information perserving analysis tools to super charge efforts.